NCMSI - MALDI-MSI Instruments

The NCMSI platform is one of the largest and most modern MALDI-MSI laboratories in Europe. It has two MALDI TOF-TOF mass spectrometers (Ultraflextreme, Bruker Daltonics; installed in 2012 and 2014) and two MALDI-q-TOF instruments – a Synapt G2-S HDMS with ion mobility separation (Waters Corp., installed in 2015) and a Maxis Impact mass spectrometer (Bruker Daltonics, installed in 2012). The laboratory also has several MALDI matrix application instruments including two ImagePrep instruments (Bruker Daltonics; uses vibrational vaporization technology), one CHIP-1000 Chemical Printer (Shimadzu; uses piezoelectric spotting technology), and one TM-Sprayer Tissue MALDI Sample Prep System (HTX Technologies; uses heated spray technology).

Mass Spectrometers

MALDI-TOF-TOF ultrafleXtreme, Bruker Daltonics

The ultrafleXtreme instruments can perform 2 kHz speed in MS mode and 1 kHz in MS/MS mode with ultrahigh performance and flexibility for a broad variety of MALDI-MSI applications. Can analyze samples at near cellular spatial resolution (10-20µm). Requires tissue sections to be placed on conductive glass slides. Instruments installed 2012 and 2014.

MALDI/ESI Q-Tof Synapt G2si, Waters Corp.

The MALDI SYNAPT G2-Si is powered by a solid-state laser with a repetition rate of 2.5 kHz to maximize the speed of the analysis. In addition, SYNAPT is well suited for analysis of tissue sections placed on regular microscope glass slides (non-conductive). Spatial resolution of 50-10µm. Instrument installed 2015.

ESI Q-Tof Maxis Impact, Bruker Daltonics

The instrument can produce >50,000 full-sensitivity resolution. It has high dynamic range and five orders of magnitude is achieved at UHPLC speeds from 50 GBit/sec using sampling technology. Instrument installed 2012.

ESI LTQ (ion trap) MS, ThermoScientific Corp.

This instrument has MSn performance. It was installed 2004.

Matrix Application and Tissue Cutting Instruments

TM-Sprayer, HTX-Technologies

The TM-Sprayer provides heat to the matrix solution to accelerate adsorption into the tissue, as well as a controlled flow of dry air or nitrogen to focus the spray and control drying time.

ImagePrep, Bruker Daltonics

Using vibrational vaporization technology, ImagePrep provides reproducible matrix preparations for MALDI-MSI with a fully automated process.

CHIP-1000 Chemical Printer, Shimadzu Corp.

This instrument delivers picoliter volumes of reagent to a tissue section surface using piezoelectric technology. To support the visualization of the droplet and the area of interest, the system has a camera and an in built scanner.

Leica CM1900 Cryostat, Leica Microsystems

The Leica CM1900 instrument is a rapid sectioning cryostat for advanced cutting of small animal organs.