Bioscience seminar series
- Datum: –09.45
- Plats: Join Zoom Meeting https://uu-se.zoom.us/j/63744028735
- Föreläsare: Elva Friðjónsdóttir, PhD student, Medical mass spectrometry imaging, Department of Pharmaceutical Biosciences.
- Kontaktperson: Anna Nilsson
Investigating neurochemical changes associated with Parkinson’s disease and L-DOPA-induced dyskinesia using mass spectrometry imaging
Parkinson’s disease (PD), caused by a loss of midbrain dopamine neurons, is the second most common neurodegenerative disease worldwide after Alzheimer’s disease. The primary treatment choice for PD is L-DOPA, the precursor for dopamine, which only affects symptoms and does not inhibit disease progression. Most patients develop motor complications during long-term L-DOPA treatment called L-DOPA-induced dyskinesia (LID), which are abnormal involuntary movements. LID has been associated with biochemical alterations in a number of signalling systems in the basal ganglia, including the dopaminergic, serotonergic, cholinergic and opioidergic systems, among others.
Mass spectrometry imaging enables on-tissue mapping of a large number of molecules in a single tissue section but detection of low abundant endogenous neurotransmitters and neuropeptides is challenging. We have developed methods to overcome this challenge using on-tissue chemical derivatisation and tissue clean-up procedures that improve limit of detection of multiple signalling molecules. Applying this technique to the gold-standard model of Parkinson’s disease and LID, namely the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administered non-human primate model, enabled identification of brain region specific alterations related with LID. These include that LID was associated with extremely high levels of L-DOPA, increased levels of signalling neuropeptides in the basal ganglia and increased abundance of the vasculature marker heme B in the striatum, among others. In conclusion, mass spectrometry imaging enabled investigation of multiple signalling molecules in single tissue sections and novel and comprehensive insight into the biochemical changes that occur in LID.
Presentation will be held in English